Sarah Fullington (graduate student)


joined Bretz Group in October 2017
hometown: Buffalo, NY

B.S. University of Buffalo, 2016
Ph.D. Candidate (expected graduation May 2021)

fullinsa@miamioh.edu

Dissertation Research: Investigating Student Conceptions of Ability in the Laboratory

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Cognate Research: Using Thiorphans with NDM-1 as Inhibitors of Metallo-ß-Lactamases: Discovering the Mechanism of Inhibition

This bench biochemistry project investigates using a thiol-containing approved drug Thiorphan, as a potential inhibitor of Metallo-β-Lactamases (MBL) to restore the effectiveness of β-lactam antibiotics. MBLs inactivate nearly all β-lactam containing antibiotics, which make up more than 50% of available antibiotics. There currently are no clinically approved MBLs inhibitors for clinical trials, so by determining the mechanism of inhibition of metal-targeting inhibitors like Thiorphan using NDM-1, inhibitors can be redesigned to be more selective. The mechanism of inhibition will be investigated by pre-steady state kinetic studies to probe the kinetic mechanisms of these enzymes and crystallographic and spectroscopic studies, such as NMR, EPR, UV-Vis, to probe the structure. The metal content of the MBL will be determined through ICP-AES. (image from Shen et al., PLOS, 2013.)

© 2018   Stacey Lowery Bretz   Miami University   Department of Chemistry & Biochemistry   Oxford, OH 45056   bretzsl@miamioh.edu   513.529.3731