Miami University NMR/MS Facility

Miami has seven NMR instruments (6 Bruker, 1 Varian), spanning 200 MHz to 850 MHz. Department-wide access is available on three instruments: a 200 MHz primarily used for undergraduate teaching, a 300 MHz primarily used for 1H NMR, and a 500 MHz for 2D NMR and all nuclei (except 19F). NMR "walk-up" time is available weekdays from 10-2 PM: on M/W/F the walk-up is on the 500 MHz, on T/Th it is on the 300 MHz.

Three shared mass spectrometers are also available; the MALDI-TOF offers excellent sensitivity and resolution for biomolecules and polymers, the ESI instrument for small molecules, and the ICP for quantification of metals.

NMR Instruments

Bruker DPX200 (Liquids, Hughes Room 86), VT: ~170 K - 370 K
- Default Probe: 5 mm QNP (1H, 13C, 31P, 19F)

Bruker DPX300 (Liquids, Hughes Room 63), VT: ~170 K - 370 K
- Default Probe: 5 mm BBI (1H/19F inner, broadband outer coil)
- Alternate Probe: 5 mm BBO (1H outer, broadband inner coil)

Bruker AV500 (Liquids, Hughes Room 65), VT: ~270 K - 370 K
- Probes: 5 mm BBO with Z-gradients(1H outer coil, broadband inner coil)
                5 mm TXI with Z-gradients(1H inner coil, 13C, 15N outer coil)

Bruker AV500WB (Solids, Hughes 126)
- Default Probe: 4 mm HX CP/MAS (13 kHz max. speed)
- Alternate probe(s): various flat cell and static probes
- Exclusive to the Lorigan research group

Bruker AVIII600 (Liquids, Hughes Room 23), VT: ~270 K - 370 K
- Default Probe: 5 mm TXI with Z-gradients(1H inner coil, 13C, 15N outer coil)

Varian Inova600 (Liquids, Hughes Room 23), VT: ~270 K - 350 K
- Default Probe: 5 mm HCN (1H inner coil, 13C, 15N outer coil)
- Alternate Probe: 5 mm cold probe (1H inner coil, 13C, 15N outer coil)
- Exclusive to the Kennedy research group

Bruker AVIII850 (Liquids, Hughes Room 23)
- Default Probe: 5 mm TXI with Z-gradients(1H inner coil, 13C, 15N outer coil)

Mass Spec Instruments

Bruker Autoflex III TOF/TOF (Hughes Room 76)
- MALDI-TOF instrument with Smartbeam (355 nm) laser, 200 Hz repetition rate
- Equipped with LIFT cell for MS/MS experiments

Bruker Esquire-LC (Hughes Room 76)
- ESI instrument with ion trap
- Capable of MSn (up to 10)
- Direct injection mode only

Varian "Red Top" ICP-MS
- ICP instrument with single quadrupole mass separation
- attached to an autosampler