Miami has seven NMR instruments (6 Bruker, 1 Varian), spanning 200 MHz to 850 MHz. Department-wide access is available on three instruments: a 200 MHz primarily used for undergraduate teaching and for 1H and 19F NMR, and a 500 MHz for 2D NMR and all nuclei (except 19F). NMR "walk-up" time is available weekdays: on M-F 1-3 PM on the 500 MHz and T/Th 11-1 PM on the 300 MHz.
Three shared mass spectrometers are also available at the MS Facility; a Bruker Esquire-LC, a Maldi Autoflex III TOF/TOF, and the LTQ Orbitrap XL.
NMR Instruments
Bruker DPX200 (Liquids, Hughes Room 86), VT is not available
- Default Probe: 5 mm QNP (1H, 13C, 31P, 19F)
Decommissioned in Jan. 2020:
Bruker DPX300 (Liquids, Hughes Room 63), VT: ~175 K - 373 K
- Default Probe: 5 mm BBI (1H/19F inner, broadband outer coil)
- Alternate Probe: 5 mm BBO (1H outer, broadband inner coil)
Installed in March, 2020:
Bruker Avance NEO 400i nanobay (Liquids, Hughes 63), VT -150 C - 150 C
-Probe: SmartProbeTM BBFO (1H outer, 19F and broadband inner coil)
-SampleCase autosampler
Bruker AV500 (Liquids, Hughes Room 65), VT: ~190 K - 373 K
- Probes: 5 mm BBO with Z-gradients(1H outer coil, broadband inner coil)
5 mm TXI with Z-gradients(1H inner coil, 13C, 15N outer coil)
Bruker AV500WB (Solids, Hughes 126)
- Default Probe: 4 mm HX CP/MAS (13 kHz max. speed)
- Alternate probe(s): various flat cell and static probes
- Exclusive to the Lorigan research group
Bruker AVIII600 (Liquids, Hughes Room 23), VT: ~270 K - 370 K
- Default Probe: 5 mm TXI with Z-gradients(1H inner coil, 13C, 15N outer coil)
Varian Inova600 (Liquids, Hughes Room 23), VT: ~270 K - 350 K
- Default Probe: 5 mm HCN (1H inner coil, 13C, 15N outer coil)
- Alternate Probe: 5 mm cold probe (1H inner coil, 13C, 15N outer coil)
- Exclusive to the Kennedy research group
Bruker AVIII850 (Liquids, Hughes Room 23)
- Default Probe: 5 mm TXI with Z-gradients(1H inner coil, 13C, 15N outer coil)
Mass Spec Instruments
Bruker Autoflex III TOF/TOF (Hughes Room 76)
- MALDI-TOF instrument with Smartbeam (355 nm) laser, 200 Hz repetition rate
- Both positive and negative mode MS
- Equipped with LIFT cell for MS/MS experiments
- Capabilities include:
* Reflector mode for high-resolution MS of small molecules and peptides (< 3500 m/z)
* Confirmation or identification of small molecules and polymers
* Organometallic salts
* Linear mode for proteins as large as 130 kDa.
* Trypsin digests can be run and peptides identified and mapped using MASCOT software. This can be used to confirm the identity of a single protein cut from a gel.
Bruker Esquire-LC ESI MS (Hughes Room 76)
- ESI instrument with ion trap
- Capable of MSn (up to 10)
- Direct injection mode only
- Mass range is 15 - 2200 m/z.
- Common applications include:
* Confirmation or identification of small molecules and polymers
* Full length protein size determination by deconvolution of multiply charged peak patterns
* Soft ionization methods can be used for observation of metal-ligand complexes.
Thermo Scientific LTQ Orbitrap XL Mass Spectrometer (Hughes Room 76)
-Can be run with direct infusion for coupled to either of these UHPLCs:
1. Thermo Scientific DIONEX UltiMate 3000 RSLC, autosampler, variable wavelength detector.
2. EASY-nLC 1000 from ThermoFisher (for proteomics only)
- Hybrid ESI instrument with both an ion trap and orbitrap MS.
- Capable of MSn in linear ion trap (LTQ).
- high resolution with the Orbitrap XL detector; with resolution up to 120,000 FWHM and mass accuracy < 3 ppm.
- Common applications include:
* Proteomics, metabolomics, identification and quantitation of unknown targets
* Accurate mass MS data for synthesized molecules required for publication.
* Native protein MS capable with nanoSpray adaptor (ES260). This can be used to detect ligand- and metal-bound proteins.
For more information on how the Miami University NMR/MS Facility can support your research, please contact the NMR and MS Specialist at nmr.ms@miamioh.edu.