Miami University NMR/MS Facility

Training is a necessary first step in gaining access to the facility's equipment. We offer training for general NMR (e.g. the 200 MHz, 300 MHz, and 500 MHz systems), high-field biomolecular NMR, and MALDI-TOF mass spectrometry.

All users must be trained by the instrumentation specialist. Users are not permitted to train one another, under any circumstances.

Sessions are held at the beginning of each semester, but can also be arranged at any other time as needed by e-mail.

NMR Training

Miami uses a 'graduated' NMR training program. The first step is a 90-minute hands-on training session on collecting 1H spectra using the 200/300 MHz NMR instruments. The next step is a "driver's test", where users demonstrate independent operation of the 200/300 MHz NMR in safe manner. After passing, users will be able to use these NMRs independently and book time as needed.

If access to the 500 MHz NMR is desired, the next step is a 60-minute training session (covering both 1H and 13C spectra) followed by another "driver's test". Further training on advanced topics such as 2D NMR, variable temperature NMR, or other nuclei (e.g. 19F, 31P) is readily arranged on a one-on-one basis.

Biomolecular NMR Training

Once your supervisor has arranged access to the 600 MHz or 850 MHz NMR spectrometers, a one-on-one training session can be arranged. Typical sessions cover sample preparation, NMR setup (locking, tuning, shimming, VT, and pulse calibrations), and the collection of 1D WATERGATE and 2D HSQC spectra. Other topics (e.g. backbone assignment, T1/T2/NOE or CPMG dynamics, NOESY spectra) can be covered on request.

MALDI-TOF Training

Training on the MALDI-TOF instrument is a 90-minute hands-on session, covering sample and matrix preparation, spotting techniques, data collection, and analysis. After training, users will be able to operate the MALDI-TOF independently and book time as needed.